IN-FRAME DELETION OF ESCHERICHIA COLI ESSENTIAL GENES IN COMPLEX REGULON

A conditional knockout-rescue system was developed to construct an Filling Machine Accessory in-frame deletion strain of Escherichia coli essential genes.The target was flanked with marker genes and FRT (FLP recognition target) sites, and a plasmid containing arabinose-induced FLP recombinase was transformed.After arabinose induction, cells could s

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